DOT Drug & Alcohol Testing

DOT Drug & Alcohol Testing: What Employers Must Know in 2025

The Department of Transportation (DOT) requires strict drug and alcohol testing for all employees performing safety-sensitive duties. Whether your business operates in transportation, oil & gas, aviation, pipeline, rail, or maritime work, DOT compliance is mandatory — not optional.

According to the DOT Guide “What Employers Need to Know About DOT Drug and Alcohol Testing — Guidance and Best Practices”, employers must maintain a written policy, identify covered employees, and administer required testing: pre-employment, random, post-accident, reasonable suspicion, return-to-duty, and follow-up testing.

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DOT testing screens for marijuana, cocaine, amphetamines, opioids, and PCP using only urine or oral fluid — no hair or instant tests are allowed. Oral fluid testing is now permitted but must be directly observed and included in your written policy.

If an employee violates a rule, employers must immediately remove them from safety-sensitive duties and refer them to a qualified Substance Abuse Professional (SAP). Employers under FMCSA must also report violations to the Clearinghouse.

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Staying compliant protects your workforce, the public, and your business from costly penalties.
TAF Diagnostics Services supports Odessa and Midland employers with DOT testing, mobile collections, breath alcohol testing, and compliance support.

To set up a compliant Employer Testing Program, call (432) 315-2390 or email [email protected].